The team of Prof. Karl Kunzelmann and that of Prof. Margarida Amaral at the Faculty of Sciences / University of Lisbon collaborate closely a productive lab with an international team. Join them!
- CFTR and anoctamins in cystic fibrosis
Lung disease are very common and often are the airways affected. Human airways express important ion channels such as CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Cl- channels, Ca2+ activated Cl- channels (Anoctamins), and Cl- channels regulated by cell volume (LRRC8). Cl- channels are essential for electrolyte secretion and proper lung function. CFTR is well examined and, when mutated, causes cystic fibrosis, a severe lung and intestinal disease.
Anoctamins are very important in asthma. Although CFTR, anoctamins, and LRRC8 are independent protein entities, they demonstrate a large functional overlap. Surprisingly, transgenic mice in which anoctamins have been knocked out show a reduced CFTR-dependent transport.
Also, knockdown of anoctamins strongly inhibits LRRC8A ion transport. This project aims in understanding the correlation between CFTR and anoctamins, with the long term goal to identify novel drugs that improve lung function in cystic fibrosis and asthma.
- Standard molecular biology techniques
- Protein expression and analysis
- Videoimaging / Fluorescence based techniques
- Standard protein biochemistry and immunocytochemistry
- Transgenic animals
Why would you do science abroad?
- Join a productive lab with an international team
- Work on cutting edge scientific questions
- Enjoy a stimulating environment with excellent supervision
- Receive a fellowship
- Prof. Dr. M. Amaral, Faculdade de Ciências da Universidade de Lisboa
Campo Grande, Telefone: +351 217500000 ext. 28252
- Prof. Karl Kunzelmann